Recently, we installed Appian 16.1 on one of our server. We are getting (attache

Recently, we installed Appian 16.1 on one of our server. We are getting (attached screenshot) "Error 401 - Unauthorized" when we hit submit button with or without entering login details. We have verified all the settings against the Appian documentation. Everything looks correct.
Could anyone point out the places where we need to look to debug this issue further?
Attached a bit of the log file from when we had debugging enabled.

OriginalPostID-210053

OriginalPostID-210053

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